Lineage-specific transcription factors have long been hypothesized to cooperate to fine-tune the expression of gene networks driving cell identity. However, the failure of chromatin binding alone to adequately predict transcription factor targets has made testing this hypothesis on a genome-wide basis challenging.

In this webinar, Dr. Kristy Stengel discusses how she and her team used acute depletion of the lineage transcription factor RUNX1, coupled with nascent transcriptomic, to first define the gene targets directly regulated by RUNX1 and then identify the RUNX1-bound enhancers responsible for this regulation.